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Third, the HBP generates UDP-GlcNAc, enabling O-GlcNAcylation of various proteins (including HSF1 and components upstream of mTOR/AMPK), with implications for insulin signaling, transcription, and protein synthesis [11,12,49,50]

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Clonal expansion and characterization Clonal expansion of iPSCs involves isolating and expanding individual iPSC colonies to ensure clonality and genetic homogeneity